Determination of pesticides in hydroponic water for environmental phytoremediation

This study validated a simple and fast method by High-Performance Liquid Chromatography with Diode Array Detector (HPLC-DAD) for pesticide phytoremediation analysis.

The method was developed in water and in a hydroponic medium. Sample extraction and concentration were performed by Solid Phase Extraction (SPE) with Strata C18-E type cartridges. The SPE-HPLC-DAD method was successfully applied in the detection and quantification of quinclorac, 2,4-D, propanil, bentazon, clomazone, and tebuconazole in water and hydroponic medium for 14 days.

The method presented excellent results with the linearity of 0.9969-0.9994, and the lowest limit of detection (LOD) and quantification (LOQ) of pesticides was 1.7 μg/L and 5.0 μg/L, respectively, with RSD <11.92%. The average recovery obtained ranged from 77.62% to 109.73%, and RSD <12.70%. A Lactuca sativa species promoted phytoremediation on the 7th day for 2,4-D and tebuconazole and on the 14th day for clomazone.

Read the complete research at

Rosa, Anderson & Ramborger, Bruna & Zago, Maria & Escoto, Dandara & Denardin, Elton & Roehrs, Rafael. (2022). Determination of pesticides in hydroponic water for environmental phytoremediation. Ciência e Natura. 44. e27. 10.5902/2179460X68460. 


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